Anilocus provides bioanalytical services for preclinical research applications from our Germantown, Maryland facility. Our molecular and cellular analysis capabilities support drug development programs through quantitative assessment of biological samples and tissue specimens.
qPCR Analysis
We perform quantitative PCR analysis on tissue samples using QuantStudio 7 and ProFlex systems. Our qPCR services include gene expression analysis, viral load quantification, and copy number determination across various sample types including fresh, frozen, and FFPE tissues.
Our capabilities include custom assay development, primer design and validation, and multiplexed reactions for simultaneous analysis of multiple targets. We provide standard curve generation, efficiency calculations, and statistical analysis of expression data with appropriate controls and normalization strategies.
Western Blot and Protein Expression
Our protein analysis services utilize the FluorChem E imaging system for Western blot detection and quantification. We analyze protein expression, phosphorylation states, and post-translational modifications from tissue and cell lysates with optimized extraction protocols for different sample types.
Services include antibody validation, loading control normalization, and densitometric analysis for quantitative protein measurements. We optimize protocols for specific proteins of interest and provide troubleshooting for challenging targets or sample types.
Flow Cytometry
We perform flow cytometry analysis of immune cells and brain-resident cell populations using BD FACS Celesta and Melody systems. Our capabilities include immunophenotyping, cell viability assessment, and functional analysis of cellular responses to treatment interventions.
Our services encompass single-cell suspension preparation from various tissues, antibody panel design and optimization, and multi-parameter analysis with appropriate compensation controls. We analyze immune cell subsets, activation markers, and cytokine production with statistical analysis and population gating strategies.
Imaging-Based Cell Characterization
The Amnis ImageStream system enables simultaneous flow cytometry and fluorescence microscopy for detailed cellular analysis. This technology provides morphological assessment combined with phenotypic characterization, allowing analysis of cell shape, localization, and interaction patterns.
We perform internalization studies, nuclear translocation analysis, and cell-cell interaction assessment. The imaging flow cytometry approach provides statistical power of flow cytometry with the morphological detail of microscopy for enhanced cellular characterization.
Metabolic Profiling
Our Seahorse XFe96 system measures cellular bioenergetics in ex vivo tissue samples and isolated cells. We assess mitochondrial respiration, glycolytic function, and metabolic flexibility in response to drug treatments or disease conditions.
Metabolic profiling services include oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements, stress test protocols, and substrate utilization analysis. We provide metabolic phenotyping of different cell types and tissues with appropriate controls and standardization procedures.
Histological Evaluation
We perform histological evaluation and tissue morphology analysis using Leica Cryostat for section preparation and Olympus IX-83 microscopy systems. Our capabilities include routine histopathology, immunohistochemistry, and specialized staining techniques for tissue characterization.
Services encompass tissue processing optimization, section quality assessment, and morphometric analysis with digital pathology capabilities. We provide quantitative analysis of tissue architecture, cellular infiltration, and pathological changes with statistical assessment of treatment effects.
Sample Processing and Quality Control
Our bioanalytical services include optimized sample processing protocols for different tissue types and experimental conditions. We maintain sample integrity through appropriate storage, handling, and processing procedures with quality control measures at each step.
We provide sample volume optimization, extraction efficiency assessment, and contamination prevention protocols. Our quality control includes positive and negative controls, technical replicates, and inter-assay variability assessment to ensure reliable results.
Data Analysis and Reporting
Our bioanalytical services include statistical analysis, data visualization, and report generation tailored to client requirements. We provide raw data files, processed datasets, and summary reports with appropriate statistical methods and quality metrics.
Data analysis capabilities include fold-change calculations, pathway analysis, and correlation studies between different analytical endpoints. We maintain data integrity through version control and audit trails for regulatory compliance requirements.
Method Development and Validation
We offer method development and validation services for specialized bioanalytical applications. This includes optimization of extraction procedures, assay conditions, and detection parameters for specific research objectives and sample types.
Validation services encompass accuracy, precision, specificity, and robustness assessment with appropriate acceptance criteria. We develop standard operating procedures and provide method transfer support for client laboratories.
Integration with Study Services
Our bioanalytical capabilities integrate seamlessly with in vivo study services, providing end-to-end support from study design through final data analysis. We coordinate sample collection timing, processing requirements, and analytical schedules to optimize study outcomes.
We work with study directors to design sampling strategies that maximize data quality while minimizing animal usage. Our integrated approach ensures consistency between study conduct and analytical assessment for reliable, interpretable results.
Key Takeaways
- Anilocus provides bioanalytical services including qPCR, Western blot, flow cytometry, and metabolic profiling using specialized instrumentation and validated protocols.
- Bioanalytical studies require optimized sample processing, quality control measures, and appropriate statistical analysis for reliable data interpretation and conclusions.
- Integrated bioanalytical services accelerate preclinical research timelines by combining sample collection, processing, and analysis within single research facilities.
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Anilocus offers bioanalytical services from our Germantown, MD facility. Our molecular and cellular analysis capabilities include qPCR, Western blot, flow cytometry, metabolic profiling, and histological evaluation. Contact us through our website to discuss your bioanalytical requirements and receive a proposal for your research projects.
